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KMID : 0381120170390111227
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Genes and Genomics
2017 Volume.39 No. 11 p.1227 ~ p.1235
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Transcriptomic analysis reveals the molecular mechanism of apoptosis induced by Muscovy duck reovirus
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Wang Quanxi
Liu Mengxi
Yuan Xiaoqin
Li Chunyan
Chen Shiyi
Zhuang Yubin
Wu Yijian
Huang Yifan
Wu Baocheng
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Abstract
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This study was to investigate the molecular mechanism underlying the apoptosis induced by Muscovy duck reovirus (MDRV) through a transcriptomic analysis. Muscovy ducklings were infected with MDRV strain YB and the apoptotic cells in their livers were examined with terminal-deoxynucleotidyl-transferase-mediated nick end labeling and flow cytometry. Genes differentially expressed in the livers of the MDRV-infected ducklings were screened by comparing them with those of uninfected ducklings and were analyzed with a transcriptomic method to illuminate the mechanism of MDRV infection. The results showed that MDRV infection strongly induced apoptotic cells in liver. Significant pathway enrichment determined by a Kyoto Encyclopedia of Genes and Genomes analysis showed that MDRV activated the death receptor family signaling pathway (Fas, TNFR1), the interleukin receptor signaling pathway (IL1, IL3), the phosphatidylinositol 3-kinase signaling pathway, NF-?B signaling pathway and calcium ions signaling pathway to induce apoptosis. This was verified by SYBR-Green-based fluorescence quantitative PCR. In conclusion, MDRV induce apoptosis by activation multi signaling pathways.
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KEYWORD
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Muscovy duck reovirus, Transcriptome, Genes, Apoptosis
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